Morphologic Phenotyping with MR Microscopy: The Visible Mouse1
- G. Allan Johnson, PhD,
- Gary P. Cofer, MS,
- Sally L. Gewalt, MS and
- Laurence W. Hedlund, PhD
- 1From the Center for In Vivo Microscopy, Duke University Medical Center, Rm 141, D Bryan Neuroscience Bldg, Research Dr, Durham, NC 27710. Received March 5, 2001; revision requested April 14; final revision received September 5; accepted September 6. Supported by the National Institutes of Health National Center for Research Resources (P41 RR05959). Address correspondence to G.A.J. (e-mail: gaj@orion.mc.duke.edu).
Abstract
A method for rapid morphologic phenotyping is demonstrated by using magnetic resonance microscopy. Whole fixed C57BL/6J mice were imaged at 110-μm isotropic resolution; limited volumes of the intact specimen, at 50-μm isotropic resolution; and isolated organs, at 25-μm isotropic resolution. The three-dimensional imaging technique was applied to uricase knockout mice to demonstrate the method for the evaluation of morphologic phenotype.
Supplemental material: (radiology.rsnajnls.org/cgi/content/full/2223010531/DC1)
© RSNA, 2002
Footnotes
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Abbreviations: TE = echo time, TR = repetition time, 3D = three-dimensional
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Author contributions: Guarantor of integrity of entire study, G.A.J.; study concepts and design, G.A.J., L.W.H., S.L.G., G.P.C.; literature research, G.A.J.; experimental studies, G.A.J.; data acquisition, G.A.J., L.W.H., G.P.C.; data analysis/interpretation, G.A.J., L.W.H.; manuscript preparation, G.A.J., L.W.H., S.L.G., G.P.C.; manuscript definition of intellectual content, G.A.J.; manuscript editing, G.A.J., L.W.H., S.L.G.; manuscript revision/review and final version approval, G.A.J., L.W.H., S.L.G., G.P.C.
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G.A.J. is a cofounder of MRPath, formed to commercialize the application of magnetic resonance histology. L.W.H. and S.L.G. have consulted for the company.
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